K 252a br H Beitollahi M Safaei M
 H. Beitollahi, M. Safaei, M.R. Shishehbore, S. Tajik, Application of [email protected]/GO nanocomposite for sensitive and selective electrochemical sensing of tryptophan, J. Electrochem. Sci. Eng. 9 (2019) 45–53.  S.Z. Mohammadi, H. Beitollahi, S. Tajik, Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine, Micro Nano Syst. Lett. 6 (2018) 9.
Dan Ou is presently pursuing her master degree in pharmaceutical analysis at Sun Yat-Sen University. Her current research focuses on electrochemical biosensor and its appli-cations in cancer cell research and drug evaluation.
Duanping Sun received his Ph.D. in pharmaceutical analysis at Sun Yat-Sen University in 2016 under the supervision of Professor Zuanguang Chen. From 2016 to 2018, he worked as a research associate in School of Pharmaceutical Sciences, Sun Yat-Sen University. Currently, he is employed as an associate professor in Center for Drug Research and Development, Guangdong Pharmaceutical University. His current research interests focus on electrochemical biosensor and cell-based drug screening.
Zuanguang Chen received his B.S. and Ph.D. in analytical chemistry at Sun Yat-Sen University in 1982 and 2000, respectively. As a professor and director of the Institute of Medical Instrument and Application, School of Pharmaceutical Sciences, Sun Yat-Sen University, his current research interests include analytical instruments, microfluidic system, electrochemical sensor, and pharmaceutical analysis.
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European Journal of Pharmacology
journal homepage: www.elsevier.com/locate/ejphar
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A novel derivative of artemisinin inhibits cell proliferation and K 252a T via down-regulation of cathepsin K in breast cancer
a State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China b Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China
Breast cancer is one of the main diagnosis cancers annually worldwide. It is difficult to thorough cure due to drug resistance and the high possibility of metastasis. SM934 is a novel water-soluble artemisinin analog, and has been reported to have a promising therapeutic effect on multiple autoimmune diseases. In this study, SM934 was combined with Testosterone, which is related to prostate cancer, and the reaction product was SM934-Testosterone. We aimed to explore whether SM934, Testosterone and SM934-Testosterone could inhibit tu-morigenesis and metastasis of breast cancer cells. Moreover, the mechanism also remains to be clarified. The results of our study showed that among the three compounds, only SM934-Testosterone treatment could lead to the suppression of cell proliferation and metastasis with IC50 = 30.66 ± 2.13 μM at 24 h in MDA-MB-231 and IC50 = 31.11 ± 1.79 μM at 24 h in SK-BR-3, where apoptosis was induced. But SM934-Testosterone showed little effects on breast cancer in vivo due to its poor water-solubility. Furthermore, computational target pre-diction and experimental validation demonstrated that Cathepsin K was the target of SM934-Testosterone. SM934-Testosterone inhibited the expression of Cathepsin K in breast cancer cells. Then, down-regulation of Cathepsin K in cancer cells by transfected with Cathepsin K shRNA inhibited cell proliferation and metastasis of breast cancer cells. Moreover, pathway enrichment was performed to understand the mechanism of action that Cathepsin K could adjust apoptosis regulator Bcl-X, and knockdown of Cathepsin K by SM934-Testosterone resulted in the reduction of Bcl-xL, which has been reported to be related to the proliferation and metastasis of cells. Collectively, SM934-Testosterone inhibited proliferation and metastasis ability of breast cancer cells via inhibiting the expression of Cathepsin K followed by the inhibition of Bcl-xL.